1. What are the features of the PURE™ series that make it different from other extraction products?
A : Other products use pretreatment(shredder) columns and require two types of spin columns, resulting in a long or complicated extraction process. In addition, some products use organic solvents such as phenol and chloroform. The PURE™ series developed by Infusion Tech does not use pretreatment columns and enables high-purity, high-concentration nucleic acid purification with a single spin column. The precipitation process using organic solvents is also not required, making it a quick and easy three-step process (lysis, wash, elute).
2. What kind of samples can extraction be performed?
A : This kit is optimized for extracting from cultured cells (≤1X107 cells) and tissues(≤30mg).
3. Do I need to grind tissue samples with liquid nitrogen?
A: If the tissue size is large, it is difficult for the buffer to penetrate and lysis efficiency will be significantly reduced. Also, if liquid nitrogen is not used, degradation of RNA is likely to occur. So it is recommended to use liquid nitrogen, mortar and pestle to grind tissue and use the appropriate volume for extraction.
4. Is it possible to perform follow-up experiments (NGS, Sequencing, PCR) with the PURE™ Cell&Tissue RNA Extraction Kit?
A : PURE™ Cell&Tissue RNA Extraction Kit has the advantage of extracting high purity and high concentration of nucleic acids, so it is possible to perform subsequent experiments. Extracted RNA can be used in various experiments including NGS, sequencing, PCR, electroporation, and cloning.
5. Are the components of the PURE™ Cell&Tissue RNA Extraction Kit sterile?
A: The components of the PURE™ Cell&Tissue RNA Extraction Kit are sterile.
6. Which buffers in the PURE™ Cell&Tissue RNA Extraction Kit do I add ethanol to?
A: RB buffer and Wash buffer should be used after adding ethanol. If you want to check for other components, please refer to the MSDS of the product.
7. How is product quality control (QC) performed for the PURE™ Cell&Tissue RNA Extraction Kit?
A: The QC of all products is performed by randomly selecting products of the same LOT after production and performing extraction using our standard sample. The test methods are quantification, electrophoresis, and PCR. Products are released if they meet our criteria.
8. The filter was clogged while using it, why?
A: The amount of sample used to perform extraction is presumed to be high. We recommend re-experimenting within the sample volume range specified in the manual. If the amount of sample is small, it is likely that a low concentration will be measured when quantitating, so please reduce the amount of elution buffer.
9. There are white powder on the wall of the spin column. Is there something wrong with the product?
A : The powder on the wall of the spin column is filter powder caused by static electricity during the manufacturing process. When the solution pass through the filter, the powder will be caught by the filter, and will not influence purity of the eluted RNA.