Principles

Fusarium Oxysporum Test Kit

: The SensPERT Fusarium Oxysporum test kit is designed to detect Fusarium Oxysporum that causes fungal wilt in plant. Fusarium Oxysporum titer is measured by solid phase immunochromatographic assay for the rapid and semi-quantitative detection fungi in plant extract. Two monoclonal antibodies in the kit specifically bind with different epitopes of the antigens. After being absorbed into the cellulose pad, the antigens of Fusarium Oxysporum move and bind with gold-colloid complex of monoclonal anti-Fusarium Oxysporum of the conjugate pad, forming Ab-Ag complex. This complex, then, forms Ab-Ag-Ab direct sandwich binding with the antibody of another monoclonal anti-Fusarium Oxysporum in the nitrocellulose membrane. The test results can appear on Control and Test lines where the principles of immuno-chromatography are applied

 

Ralstonia Solanacearum Test Kit

: The SensPERT Ralstonia Solanacearum test kit is designed to detect Ralstonia Solanacearum that causes bacterial wilt in plant. Ralstonia Solanacearum titer is measured by solid phase immunochromatographic assay for the rapid and semi-quantitative detection bacteria in plant extract. The Ralstonia Solanacearum Test Device has a letter of “T” and “C” as test line and control line on the surface of the card. Both the test line and control line in result window are not visible before applying any samples. The control line is used for procedural control. Control line should be always appear if the test procedure is performed properly and the test reagents of control line are working. A purple test line will be visible in the result window if there are enough Ralstonia Solanacearum antigen in the specimen. The specially selected antibodies are used in test band as both capture and detector materials. These enable the device to identify Ralstonia Solanacearum antigen in plate extract with a high degree of accuracy.

 

Characteristics

1) One-step rapid test of Fusarium Oxysporum & Ralstonia solanasearum

2) Rapid test results between 10 minutes

3) Expensive equipment not required

4) Easy storage and maintenance

5) High-purity and high-quality materials of the test kit increases its sensitivity and Specificity

 

Composition

Specimen well (S: for dropping), Test line (T), and Control line (C) are marked on the device. Inside of it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test paper) and absorbent pad

 

Uses

1) Specimen

Plant tissue extract

2) Test procedure

a) Collect Plant tissue sample into a sample bottle containing 5㎖ of buffer

b) Shaking Bottle for 30 seconds to 1 minutes

c) Drop 4 drops (100 ㎕) into the specimen well (S).

d) Read the test results 10 minutes.

♠ Invalid result after 10 minutes

3) Interpretation of the results

A purple band should appear on the control line regardless of the test result. The presence of another band on the test line determines the result

Control Line (C): The line should always appear regardless of the presence of the antigens of Fusarium Oxysporum or Ralstonia Solanacearum. If this line does not appear, the test should be considered invalid. This is might be because of impure buffer or the lack of specimen. It should be tested again with another kit..

Test Line (T): The presence of Fusarium Oxysporum and /or Ralstonia Solanacearum determines the test line.

1) Fusarium Oxysporum Interpretation